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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)

Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14399] to SH3PX1/SNX9 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free
    See all SH3PX1/SNX9 primary antibodies
  • Description

    Rabbit monoclonal [EPR14399] to SH3PX1/SNX9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250527 is the carrier-free version of ab181856 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SH3PX1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14399
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Adapters

Images

  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)

    This data was developed using ab181856, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: SH3PX1/SNX9 knockout HAP1 cell lysate (20 µg) 

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HCT116 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181856 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181856 was shown to specifically react with SH3PX1/SNX9 when SH3PX1/SNX9 knockout samples were used. Wild-type and SH3PX1/SNX9 knockout samples were subjected to SDS-PAGE. ab181856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)

    This data was developed using ab181856, the same antibody clone in a different buffer formulation.

    Western blot analysis of HepG2 cell lysate immunoprecipitated using ab181856 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
  • Flow Cytometry - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Flow Cytometry - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)

    This data was developed using ab181856, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Immunocytochemistry - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Immunocytochemistry - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    This data was developed using ab181856, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
  • Immunocytochemistry - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Immunocytochemistry - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    This data was developed using ab181856, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    This data was developed using ab181856, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SH3PX1/SNX9 with ab181856 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856) at 1/10000 dilution + HCT-116 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181856, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856) at 1/50000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    This data was developed using ab181856, the same antibody clone in a different buffer formulation.

     

  • Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)
    Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (ab250527)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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