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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-D4 GDI antibody [EPR14211(B)] (ab181259)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14211(B)] to D4 GDI
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-D4 GDI antibody [EPR14211(B)]
    See all D4 GDI primary antibodies
  • Description

    Rabbit monoclonal [EPR14211(B)] to D4 GDI
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, U937, HL60 and Human bone marrow lysates; Human spleen tissue; HL60 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14211(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other

Images

  • Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/10000 dilution + Human bone marrow lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 23 kDa

  • Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)

    Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181259 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)

    Immunofluorescent analysis of HL60 cells (-20°Acetone-fixed) labeling D4 GDI with ab181259 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling D4 GDI with ab181259 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    All lanes : Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HL60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
    Anti-D4 GDI antibody [EPR14211(B)] (ab181259)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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