Anti-D4 GDI antibody (ab226350)
Key features and details
- Rabbit polyclonal to D4 GDI
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-D4 GDI antibody
See all D4 GDI primary antibodies -
Description
Rabbit polyclonal to D4 GDI -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human D4 GDI aa 50-100. The exact sequence is proprietary.
Database link: P52566 -
Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226350 was affinity purified using an epitope specific to D4 GDI immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-D4 GDI antibody (ab226350)All lanes : Anti-D4 GDI antibody (ab226350) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-D4 GDI antibody (ab226350)D4 GDI was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226350 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226350 at 0.4 µg/ml.
Lane 1: ab226350 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody (ab226350)Formalin-fixed, paraffin-embedded human stomach carcinoma inflammatory infiltrate stained for D4 GDI using ab226350 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
