Mouse MCP5 ELISA Kit (ab100723)
Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 0.41 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MCP5 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.41 pg/ml - 100 pg/mlRecovery
93 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.34 84% - 105% Serum 92.42 81% - 103% Plasma 94.14 82% - 104% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s MCP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP5 in serum, plasma and cell culture supernates.
This assay employs an antibody specific for mouse MCP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MCP5 2 vials MCP5 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MCP5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
MCP5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations -
Alternative names
- C C motif chemokine 12
- Ccl12
- MCP 1 related chemokine
see all -
Database links
- Entrez Gene: 20293 Mouse
- SwissProt: Q62401 Mouse
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MCP5 2 vials MCP5 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MCP5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
MCP5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations -
Alternative names
- C C motif chemokine 12
- Ccl12
- MCP 1 related chemokine
see all -
Database links
- Entrez Gene: 20293 Mouse
- SwissProt: Q62401 Mouse
Images
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Typical standard curve
Representative standard curve using ab100723
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Typical standard curveRepresentative standard curve using ab100723