Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse MMP8 ELISA Kit (ab206982)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 53 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse MMP8 ELISA Kit
    See all MMP8 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.5%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    53 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111 108% - 119%
    Cell culture media 100 98% - 105%
    Hep Plasma 100 95% - 103%
    EDTA Plasma 104 97% - 112%
    Cit plasma 108 105% - 113%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse MMP8 ELISA Kit (ab206982) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP8 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP8 with 53 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    MMP8 is a member of the matrix metalloproteinase family of zinc proteases that degrade extracellular matrix. MMP8 is expressed in neutrophils and is also known as neutrophil collagenase. MMP8 is expressed as a propeptide. This antibodies in this assay were raised to the mature portion of MMP8.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse MMP8 ELISA Kit (ab206982)
    Other - Mouse MMP8 ELISA Kit (ab206982)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of MMP8 standard curve.
    Example of MMP8 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of MMP8 in mouse serum, plasma (citrate), plasma (heparin) and plasma (EDTA).
    Interpolated concentrations of MMP8 in mouse serum, plasma (citrate), plasma (heparin) and plasma (EDTA).

    The concentrations of MMP8 were measured in duplicate and interpolated from the MMP8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP8 concentration was determined to be 46.5 ng/mL in mouse serum, 45.2 ng/mL in mouse plasma (Citrate), 36.6 ng/mL in mouse plasma (heparin), and 20.2 ng/mL in mouse plasma (EDTA).

  • Sandwich ELISA - Mouse MMP8 ELISA Kit (ab206982)
    Sandwich ELISA - Mouse MMP8 ELISA Kit (ab206982)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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