MMP-3 Activity Assay Kit (Fluorometric) (ab118972)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell culture supernatant, Milk, Tissue Extracts, Urine
- Sensitivity: 0.05 mU/well
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Mca Standard (1mM) Yellow 1 x 20µl MMP-3 Assay Buffer WM 1 x 25ml MMP-3 Positive Control (lyophilized) Green 1 vial MMP-3 Substrate Red 1 x 200µl -
Research areas
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all
Images
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Functional Studies - MMP-3 Activity Assay Kit (ab118972)MMP-3 Positive Control
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Functional Studies - MMP-3 Activity Assay Kit (ab118972)MMP-3 Mca Standard