Human MMP8 Matched Antibody Pair Kit (ab220120)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 31.2 pg/ml - 2000 pg/ml
Overview
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Product name
Human MMP8 Matched Antibody Pair Kit
See all MMP8 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
7.1 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Mouse MMP8 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MMP8.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219050. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human MMP8 Capture Antibody 2 x 50µg 1 x 50µg Human MMP8 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human MMP8 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Can degrade fibrillar type I, II, and III collagens. -
Tissue specificity
Neutrophils. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules. - Information by UniProt
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Alternative names
- CLG 1
- CLG1
- Collagenase 1
see all -
Database links
- Entrez Gene: 4317 Human
- Omim: 120355 Human
- SwissProt: P22894 Human
- Unigene: 161839 Human
Images
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Human MMP8 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human MMP8 Matched Antibody Pair Kit (ab220120)To learn more about the advantages of recombinant antibodies see here.
