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Human MMP8 ELISA Kit (ab219050)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 19.5 pg/ml
  • Range: 78 pg/ml - 5000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human MMP8 ELISA Kit
    See all MMP8 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 1.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 12.2%

  • Sample type

    Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    19.5 pg/ml

  • Range

    78 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 99.7 86% - 114%
    Serum 84 81% - 86%
    Hep Plasma 86 82% - 90%
    EDTA Plasma 82 79% - 85%
    Cit plasma 85 83% - 87%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human MMP8 ELISA Kit (ab219050) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP8 protein in cit plasma, edta plasma, hep plasma, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP8 with 19.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Matrix metalloproteinase-8 (MMP8), also known as neutrophil collagenase and PMNL collagenase, is a zinc and calcium binding protein encoded by the gene MMP-8. MMP8 belongs to the matrix metalloproteinase family which are involved in the degradation of extracellular matrix, and are involved in embryonic development, reproduction, and tissue remolding. MMP8 is stored inactive in granules within neutrophils, and upon secretion is activated by cleavage of a peptide through the dissociation of a conserved cysteine from a zinc ion. MMP8 degrades type I, II, and III collagens, and the protein contains four hemopexin regions.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human MMP8 ELISA Kit (ab219050)
    Other - Human MMP8 ELISA Kit (ab219050)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human MMP8 standard curve in Sample Diluent NS.
    Example of human MMP8 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Human MMP8 standard curve comparison data
    Human MMP8 standard curve comparison data

    Standard curve comparison between human MMP8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Interpolated concentrations of native MMP8 in human saliva and synovial fluid samples.
    Interpolated concentrations of native MMP8 in human saliva and synovial fluid samples.

    The concentrations of MMP8 were measured in duplicate, interpolated from the MMP8 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 37.5%, synovial fluid 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP8 concentrations were determined to be 15.4　ng/mL in saliva and 19.2 ng/mL in synovial fluid.

  • Interpolated concentrations of spiked MMP8 in human serum, plasma (citrate), plasma (EDTA), and plasma (heparin) samples.
    Interpolated concentrations of spiked MMP8 in human serum, plasma (citrate), plasma (EDTA), and plasma (heparin) samples.

    The concentrations of MMP8 were measured in duplicate, interpolated from the MMP8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1.25%, plasma (citrate) 1.25%, plasma (EDTA) 1.25%, plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Native signal was undetectable in null-spiked samples.

  • Sandwich ELISA - Human MMP8 ELISA Kit (ab219050)
    Sandwich ELISA - Human MMP8 ELISA Kit (ab219050)
    To learn more about the advantages of recombinant antibodies see here.

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