Anti-ARID1A antibody (ab272905)
Key features and details
- Rabbit polyclonal to ARID1A
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARID1A antibody
See all ARID1A primary antibodies -
Description
Rabbit polyclonal to ARID1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ARID1A (C terminal). The exact sequence is proprietary.
Database link: O14497 -
Positive control
- WB: HepG2, A549, HEK-293T, A431, HeLa cells. ICC/IF: HeLa cells. IHC-P: Human endometrial carcinoma tissue, human cervical carcinoma tissue. IP: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 2.42% Tris, 1.5% Glycine, 0.04% EGTA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody (ab272905)
4% paraformaldehyde-fixed HeLa cells stained for ARID1A (Green) using ab272905 at 1/500 dilution in ICC/IF analysis.
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Western blot - Anti-ARID1A antibody (ab272905)All lanes : Anti-ARID1A antibody (ab272905) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear extract cell lysate
Lysates/proteins at 30 µg per lane.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (ab272905)Paraffin embedded human endometrial carcinoma tissue stained for ARID1A using ab272905 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-ARID1A antibody (ab272905)Anti-ARID1A antibody (ab272905) at 1/1000 dilution + A549 cell lysate at 30 µg
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (ab272905)Paraffin embedded human cervical carcinoma tissue stained for ARID1A using ab272905 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-ARID1A antibody (ab272905)Anti-ARID1A antibody (ab272905) at 1/1000 dilution + HEK-293T cell lysate at 30 µg5% SDS-PAGE
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Western blot - Anti-ARID1A antibody (ab272905)All lanes : Anti-ARID1A antibody (ab272905) at 1/1000 dilution
Lane 1 : HEK-293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
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Immunoprecipitation - Anti-ARID1A antibody (ab272905)HepG2 whole cell extract immunoprecipitation analysis.
A: Control with 4 μg of preimmune Rabbit IgG
B: Immunoprecipitation of ARID1A protein with 4 μg of ab272905.5 % SDS-PAGE. The immunoprecipitated ARID1A protein was detected using ab272905 at 1/500 dilution.