Anti-NCOA4 antibody [439CT10.1.2] (ab62495)
![Anti-NCOA4 antibody [439CT10.1.2] (ab62495)](https://www.abcam.com/ps/products/62/ab62495/Images/ab62495-75831-anti-ncoa4-antibody-439ct1012-western-blot.jpg "Anti-NCOA4 antibody [439CT10.1.2] (ab62495)")
Key features and details
- Mouse monoclonal [439CT10.1.2] to NCOA4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-NCOA4 antibody [439CT10.1.2]
See all NCOA4 primary antibodies -
Description
Mouse monoclonal [439CT10.1.2] to NCOA4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NCOA4 aa 400 to the C-terminus (C terminal).
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Positive control
- This antibody gave a positive signal in HEK293 and PC3 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
439CT10.1.2 -
Myeloma
Sp2/0 -
Isotype
IgM -
Research areas
Images
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Western blot - Anti-NCOA4 antibody [439CT10.1.2] (ab62495)All lanes : Anti-NCOA4 antibody [439CT10.1.2] (ab62495) at 1/100 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using 3% milk as the blocking agent.
