Human ICOS Ligand ELISA Kit (B7-H2) (ab272475)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.8 pg/ml
- Range: 109 pg/ml - 7000 pg/ml
- Sample type: Cell culture media, Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ICOS Ligand ELISA Kit (B7-H2)
See all ICOS Ligand/ICOSL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.9% Inter-assay Sample n Mean SD CV% Extract 4 4.9% -
Sample type
Cell Lysate, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
21.8 pg/ml -
Range
109 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 107 102% - 111% Cell culture media 100 94% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ICOS Ligand ELISA kit (ab272475) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ICOS Ligand protein in cell extract and culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ICOS Ligand with 21.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Inducible T Cell Co-Stimulator Ligand (ICOSLG), also known as CD275 and B7-H2, is a glycoprotein encoded by the gene ICOSLG. ICOS Ligand is a single pass type I membrane protein, which consists of a 238 -amino acid extracellular portion, a 21- amino acid transmembrane region, and a 25-amino acid cytoplasmic region. ICOS Ligand is constitutively expressed in many tissues and is enhanced by treatment with TNF in peripheral blood B-cells and monocytes. ICOS acts as a costimulatory signal for T-cell proliferation and cytokine secretion. ICOS Ligand induces B-Cell proliferation and differentiation into plasma cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ICOS Ligand (B7-H2) Capture Antibody 1 x 600µl 10X Human ICOS Ligand (B7-H2) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human ICOS Ligand (B7-H2) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
Tissue specificity
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- B7 H2
- B7 homolog 2
- B7 homologue 2
see all -
Database links
- Entrez Gene: 23308 Human
- Omim: 605717 Human
- SwissProt: O75144 Human
- Unigene: 14155 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ICOS Ligand standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The ICOS Ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ICOS Ligand in human HEK293 overexpression extract based on a 5 µg/mL extract load.The concentrations of ICOS Ligand were measured in duplicate and interpolated from the ICOS Ligand standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human ICOS Ligand ELISA Kit (B7-H2) (ab272475)To learn more about the advantages of recombinant antibodies see here.
