Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MEF2B antibody [EPR22193-78] (ab242059)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22193-78] to MEF2B
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-MEF2B antibody [EPR22193-78]
See all MEF2B primary antibodies
Description

Rabbit monoclonal [EPR22193-78] to MEF2B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi, Ramos and Raji whole cell lysate. IHC-P: Human tonsil, colon and diffuse large B-cell lymphoma tissues. Flow Cyt: Daudi cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22193-78
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID: 26089142; PMID: 8668199). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)

Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID: 26089142). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID: 23974956; PMID: 26089142). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-MEF2B antibody [EPR22193-78] (ab242059)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma cell line) cell line labeling MEF2B with ab242059 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-MEF2B antibody [EPR22193-78] (ab242059)

All lanes : Anti-MEF2B antibody [EPR22193-78] (ab242059) at 1/1000 dilution

Lane 1 : Daudi (human Burkitt's lymphoma cell line), whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line), whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 39 kDa

Exposure time: 3 seconds

The 40-kDa target band is consistent with what has been described in the literature (PMID: 23974956). Minor bands below 40kDa are undetermined proteins, likely to be truncated MEF2B (PMID: 23974956).

Negative control: Hela (PMID: 8798771).

Blocking/diluting buffer and concentration: 5% NFDM/TBST
Anti-MEF2B antibody [EPR22193-78] (ab242059)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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