Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEF2B antibody [EPR22193-78] (ab242059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22193-78] to MEF2B
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MEF2B antibody [EPR22193-78]
    See all MEF2B primary antibodies

  • Description

    Rabbit monoclonal [EPR22193-78] to MEF2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi, Ramos and Raji whole cell lysate. IHC-P: Human tonsil, colon and diffuse large B-cell lymphoma tissues. Flow Cyt: Daudi cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID: 26089142; PMID: 8668199). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID: 26089142). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] (ab242059)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID: 23974956; PMID: 26089142). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MEF2B antibody [EPR22193-78] (ab242059)
    Flow Cytometry - Anti-MEF2B antibody [EPR22193-78] (ab242059)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma cell line) cell line labeling MEF2B with ab242059 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-MEF2B antibody [EPR22193-78] (ab242059)
    Western blot - Anti-MEF2B antibody [EPR22193-78] (ab242059)
    All lanes : Anti-MEF2B antibody [EPR22193-78] (ab242059) at 1/1000 dilution

    Lane 1 : Daudi (human Burkitt's lymphoma cell line), whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma cell line), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma cell line), whole cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 39 kDa


    Exposure time: 3 seconds


    The 40-kDa target band is consistent with what has been described in the literature (PMID: 23974956). Minor bands below 40kDa are undetermined proteins, likely to be truncated MEF2B (PMID: 23974956).

    Negative control: Hela (PMID: 8798771).

    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

  • Anti-MEF2B antibody [EPR22193-78] (ab242059)
    Anti-MEF2B antibody [EPR22193-78] (ab242059)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MEF2B antibody [EPR22193-78] (ab242059)

© 2021 Astana Biomed Group LLP. All rights reserved.