Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)
![Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)](https://www.abcam.com/ps/products/242/ab242332/Images/ab242332-4-242059IHC3.jpg "Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22193-78] to MEF2B - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free
See all MEF2B primary antibodies -
Description
Rabbit monoclonal [EPR22193-78] to MEF2B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, colon and diffuse large B-cell lymphoma tissue. Flow Cyt: Daudi cells.
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General notes
Ab242332 is the carrier-free version of ab242059. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242332 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22193-78 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID: 26089142; PMID: 8668199). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242059).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)](https://www.abcam.com/ps/products/242/ab242332/Images/ab242332-3-242059IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID: 26089142). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242059).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)](https://www.abcam.com/ps/products/242/ab242332/Images/ab242332-2-242059IHC1a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242059 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID: 23974956; PMID: 26089142). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242059).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)](https://www.abcam.com/ps/products/242/ab242332/Images/ab242332-1-242059FC1.jpg)
Flow Cytometry - Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma cell line) cell line labeling MEF2B with ab242059 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242059).
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![Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)](https://www.abcam.com/ps/products/242/ab242332/Images/ab242332-5-benefits-of-recombinant-antibodies.png)
Anti-MEF2B antibody [EPR22193-78] - BSA and Azide free (ab242332)
