Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MEF2B antibody [EPR22193-26] (ab242058)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22193-26] to MEF2B
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Human

Product name

Anti-MEF2B antibody [EPR22193-26]
See all MEF2B primary antibodies
Description

Rabbit monoclonal [EPR22193-26] to MEF2B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi, Ramos and Raji whole cell lysates. IP: Ramos whole cell lysate. IHC-P: Human colon, diffuse large B cell lymphoma and tonsil tissue. Flow cyt: Daudi cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22193-26
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID: 26089142; PMID: 8668199) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)

Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID: 26089142) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID: 23974956; PMID: 26089142) (PMID: 26089142) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (ab242058)

MEF2B was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab242058 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242058 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Ramos whole lysate 10 μg (Input).
Lane 2: ab242058 IP in Ramos whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242058 in Ramos whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Flow Cytometry - Anti-MEF2B antibody [EPR22193-26] (ab242058)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Daudi (human Burkitt's lymphoma cell line) cells labeling [MEF2B with ab242058 at 1/500 (red) compared with a rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-MEF2B antibody [EPR22193-26] (ab242058)

All lanes : Anti-MEF2B antibody [EPR22193-26] (ab242058) at 1/1000 dilution

Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 39 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

The 40-kDa target band is consistent with what has been described in the literature (PMID: 23974956). Minor bands below 40kDa are undetermined proteins, likely to be truncated MEF2B (PMID: 23974956).

Negative control: Hela (PMID: 8798771).
Anti-MEF2B antibody [EPR22193-26] (ab242058)

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