Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEF2B antibody [EPR22193-26] (ab242058)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22193-26] to MEF2B
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-MEF2B antibody [EPR22193-26]
    See all MEF2B primary antibodies

  • Description

    Rabbit monoclonal [EPR22193-26] to MEF2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi, Ramos and Raji whole cell lysates. IP: Ramos whole cell lysate. IHC-P: Human colon, diffuse large B cell lymphoma and tonsil tissue. Flow cyt: Daudi cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID: 26089142; PMID: 8668199) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID: 26089142) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (ab242058)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID: 23974956; PMID: 26089142) (PMID: 26089142) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (ab242058)

    MEF2B was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab242058 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242058 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Ramos whole lysate 10 μg (Input).
    Lane 2: ab242058 IP in Ramos whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242058 in Ramos whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Flow Cytometry - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Flow Cytometry - Anti-MEF2B antibody [EPR22193-26] (ab242058)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Daudi (human Burkitt's lymphoma cell line) cells labeling [MEF2B with ab242058 at 1/500 (red) compared with a rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Western blot - Anti-MEF2B antibody [EPR22193-26] (ab242058)
    All lanes : Anti-MEF2B antibody [EPR22193-26] (ab242058) at 1/1000 dilution

    Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 39 kDa



    Blocking and dilution buffer:  5% NFDM/TBST.

    The 40-kDa target band is consistent with what has been described in the literature (PMID: 23974956). Minor bands below 40kDa are undetermined proteins, likely to be truncated MEF2B (PMID: 23974956).

    Negative control: Hela (PMID: 8798771).

  • Anti-MEF2B antibody [EPR22193-26] (ab242058)
    Anti-MEF2B antibody [EPR22193-26] (ab242058)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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