Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Jarid2 antibody [EPR6357(2)] - BSA and Azide free (ab251123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6357(2)] to Jarid2 - BSA and Azide free
  • Suitable for: WB, ICC/IF, ChIP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Jarid2 antibody [EPR6357(2)] - BSA and Azide free
    See all Jarid2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6357(2)] to Jarid2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, ChIPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251123 is the carrier-free version of ab192252. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251123 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-Jarid2 antibody [EPR6357(2)] - BSA and Azide free (ab251123)
    ChIP - Anti-Jarid2 antibody [EPR6357(2)] - BSA and Azide free (ab251123)

    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab192252 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192252).

  • Anti-Jarid2 antibody [EPR6357(2)] - BSA and Azide free (ab251123)
    Anti-Jarid2 antibody [EPR6357(2)] - BSA and Azide free (ab251123)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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