Native human MMP9 protein (dimer) (ab168863)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Native human MMP9 protein (dimer)
See all MMP9 proteins and peptides -
Biological activity
≥1’500mU/mg protein.
One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
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Purity
> 95 % SDS-PAGE.
>95% Western Blot. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Sequence
MSLWQPLVLV LLVLGCCFAA PRQRQSTLVL FPGDLRTNLT DRQLAEEYLY RYGYTRVAEM RGESKSLGPA LLLLQKQLSL PETGELDSAT LKAMRTPRCG VPDLGRFQTF EGDLKWHHHN ITYWIQNYSE DLPRAVIDDA FARAFALWSA VTPLTFTRVY SRDADIVIQF GVAEHGDGYP FDGKDGLLAH AFPPGPGIQG DAHFDDDELW SLGKGVVVPT RFGNADGAAC HFPFIFEGRS YSACTTDGRS DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP FTFLGKEYST CTSEGRGDGR LWCATTSNFD SDKKWGFCPD QGYSLFLVAA HEFGHALGLD HSSVPEALMY PMYRFTEGPP LHKDDVNGIR HLYGPRPEPE PRPPTTTTPQ PTAPPTVCPT GPPTVHPSER PTAGPTGPPS AGPTGPPTAG PSTATTVPLS PVDDACNVNI FDAIAEIGNQ LYLFKDGKYW RFSEGRGSRP QGPFLIADKW PALPRKLDSV FEERLSKKLF FFSGRQVWVY TGASVLGPRR LDKLGLGADV AQVTGALRSG RGKMLLFSGR RLWRFDVKAQ MVDPRSASEV DRMFPGVPLD THDVFQYREK AYFCQDRFYW RVSSRSELNQ VDQVGYVTYD ILQCPED -
Predicted molecular weight
220 kDa -
Amino acids
1 to 707 -
Additional sequence information
Isolated from stimulated human neutrophil granulocytes (buffy coat). Disulfide-bridged MMP-9 homodimer.
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Description
Native human MMP9 protein (Active)
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 0.05% Sodium azide
Constituents: 0.05% Brij, 0.06% Calcium chloride, 0.00001% Zinc chloride, 0.79% Tris HCl, 1.16% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Western blot - Native human MMP9 protein (dimer) (ab168863)All lanes : Anti-MMP9 antibody [EP1254] (ab76003) at 5 µg/ml
Lane 1 :Native human MMP9 protein (dimer) (ab168863)
Lane 2 :Native human MMP9 protein (Proenzyme, monomer) (ab157344)
Lane 3 :Recombinant Mouse MMP9 protein (ab39309)
Lysates/proteins at 0.1 µg per lane.
Secondary
Lanes 1-2 : Infrared labelled goat anti-rabbit (green) antibody at 1/20000 dilution
Lane 3 : Infrared labelled goat anti-rabbit (green) a at 1/20000 dilution
Performed under reducing conditions.
