Native human MMP9 protein (Proenzyme, monomer) (ab157344)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Native human MMP9 protein (Proenzyme, monomer)
See all MMP9 proteins and peptides -
Biological activity
≥1’400mU/mg protein.
One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
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Purity
> 95 % SDS-PAGE.
No other MMP contaminants are detectable. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Sequence
APRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGP ALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHH NITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQ FGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVP TRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTTANYDTDDRFG FCPSERLYTQDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTAN YDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDG RLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALM YPMYRFTEGPPLHKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCP TGPPTVHPSERPTAGPTGPPSAGPTGPPTAGPSTATTVPLSPVDDACNVN IFDAIAEIGNQLYLFKDGKYWRFSEGRGSRPQGPFLIADKWPALPRKLDS VFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRS GRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYRE KAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED -
Predicted molecular weight
92 kDa -
Amino acids
20 to 707 -
Additional sequence information
Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation. Mature protein without signal peptide.
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Description
Native human MMP9 protein (Proenzyme)
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 0.05% Sodium azide
Constituents: 0.05% Brij, 0.06% Calcium chloride, 0.00001% Zinc chloride, 0.79% Tris HCl, 1.16% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Western blot - Native human MMP9 protein (Proenzyme, monomer) (ab157344)All lanes : Anti-MMP9 antibody [EP1254] (ab76003) at 5 µg/ml
Lane 1 :Native human MMP9 protein (dimer) (ab168863)
Lane 2 :Native human MMP9 protein (Proenzyme, monomer) (ab157344)
Lane 3 :Recombinant Mouse MMP9 protein (ab39309)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Infrared labelled goat anti-rabbit (green) antibody at 1/20000 dilution
Performed under reducing conditions.This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour before being incubated with anti-MMP9 antibody [EP1254] (ab76003; 5 microgram per mL) at 4°C overnight. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody (diluted 1:20000) for 1 hour at room temperature before imaging.
