Anti-Hemopexin antibody (ab133415)
Key features and details
- Rabbit polyclonal to Hemopexin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Hemopexin antibody
See all Hemopexin primary antibodies -
Description
Rabbit polyclonal to Hemopexin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide corresponding to Human Hemopexin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab151532) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Sciatic Nerve; Rat Sciatic Nerve; Mouse Liver; Rat Liver.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hemopexin antibody (ab133415)All lanes : Anti-Hemopexin antibody (ab133415) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Rat Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Hemopexin has been shown to migrate at ~70 kDa under reducing conditions by Madore et al PMID: 9930744. We welcome customer feedback. Hemopexin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133415 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
