Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hemopexin antibody [EPR5610] (ab124935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5610] to Hemopexin
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Hemopexin antibody [EPR5610]
    See all Hemopexin primary antibodies

  • Description

    Rabbit monoclonal [EPR5610] to Hemopexin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Hemopexin aa 400-500. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal liver and plasma lysates. IHC-P: Human liver tissue. Flow Cyt: HepG2 cells. ICC: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)
    All lanes : Anti-Hemopexin antibody [EPR5610] (ab124935) at 1/10000 dilution

    Lane 1 : Fetal liver lysate
    Lane 2 : Human plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 52 kDa
    Observed band size: 70-75 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935) This image is courtesy of an Abreview submitted by Steffen Rickelt

    ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Immunocytochemistry - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Hemopexin with purified ab124935 at 1/200 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.8 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Flow Cytometry - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Flow Cytometry - Anti-Hemopexin antibody [EPR5610] (ab124935)

    Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)

    ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935)
    OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Hemopexin antibody [EPR5610] (ab124935)
    Anti-Hemopexin antibody [EPR5610] (ab124935)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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