Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5610] to Hemopexin - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Hemopexin antibody [EPR5610] - BSA and Azide free
    See all Hemopexin primary antibodies

  • Description

    Rabbit monoclonal [EPR5610] to Hemopexin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue. Flow Cyt: HepG2 cells. ICC: HepG2 cells

  • General notes

    Ab226099 is the carrier-free version of ab124935. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226099 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    Flow Cytometry - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

    Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099) This image is courtesy of an Abreview submitted by Steffen Rickelt

    ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935).

  • Immunocytochemistry - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    Immunocytochemistry - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    This data was developed using the same antibody clone in a different buffer formulation (ab124935).
    Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Hemopexin with purified ab124935 at 1/200 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.8 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

    ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935).

  • Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
    Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

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