Human Hemopexin ELISA Kit (ab221838)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 370 pg/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Hemopexin ELISA Kit
See all Hemopexin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.3% Inter-assay Sample n Mean SD CV% Serum 3 4.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
370 pg/ml -
Range
0.78 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 104% - 113% Serum 108 97% - 117% Hep Plasma 105 97% - 117% EDTA Plasma 94 85% - 103% Cit plasma 97 86% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Hemopexin ELISA Kit (ab221838) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemopexin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemopexin with 370 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer's disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Hemopexin Capture Antibody 1 x 600µl 10X Human Hemopexin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Hemopexin Lyophilized Native Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
Images
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Other - Human Hemopexin ELISA Kit (ab221838)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human hemopexin standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native hemopexin in human serum and plasma samplesThe concentrations of hemopexin were measured in duplicates, interpolated from the hemopexin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:40,000, plasma (citrate) 1:20,000, plasma (EDTA) 1:20,000, and plasma (heparin) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 1004 µg/mL in serum, 768 µg/mL in plasma (citrate), 875 µg/mL in plasma (EDTA) and 812 µg/mL in plasma (heparin).
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 1043 µg/mL with a range of 584 – 1316 µg/mL.
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Interpolated concentrations of native hemopexin in human HepG2 cell culture supernatant samplesThe concentrations of hemopexin were measured in duplicates, interpolated from the hemopexin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 24.3 ng/mL in HepG2 supernatant.
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Sandwich ELISA - Human Hemopexin ELISA Kit (ab221838)To learn more about the advantages of recombinant antibodies see here.
