Human Hemopexin ELISA Kit (HPX) (ab108859)
Key features and details
- Sensitivity: 0.62 ng/ml
- Range: 0.25 µg/ml - 1 µg/ml
- Sample type: Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Hemopexin ELISA Kit (HPX)
See all Hemopexin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.5% Inter-assay Sample n Mean SD CV% Overall 10.5% -
Sample type
Milk, Serum, Plasma -
Assay type
Competitive -
Sensitivity
> 0.62 ng/ml -
Range
0.25 µg/ml - 1 µg/ml -
Recovery
102 %
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Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.
A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.
Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Hemopexin (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 8ml Hemopexin Microplate (12 x 8 well strips) 1 unit Hemopexin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
Images
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Competitive ELISA - Hemopexin (ab108859
Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108859.
