Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 120 614 ₸

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Anti-CD3 epsilon antibody [EPR20752] (ab215212)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20752] to CD3 epsilon
Suitable for: IHC-P, WB, IHC-Fr, IP
Reacts with: Mouse

Product name

Anti-CD3 epsilon antibody [EPR20752]
See all CD3 epsilon primary antibodies
Description

Rabbit monoclonal [EPR20752] to CD3 epsilon
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse thymus and lymph node lysate; EL4.IL-2 whole cell lysate. IHC-P: Mouse spleen and thymus tissue. IHC-fr: Mouse spleen tissue. IP: EL4.IL-2 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20752
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [EPR20752] (ab215212)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilised mouse spleen tissue labelling CD3 epsilon with ab215212 at 1/800 dilution, foloowed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive membrane staining on cells localized in the periarterial lymphatic sheath in mouse spleen tissue section is observed (PMID: 20880086) Counterstained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-CD3 epsilon antibody [EPR20752] (ab215212)

CD3 epsilon was immunoprecipitated from 10 μg of EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate with ab215212 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215212 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution
Lane 1: EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate 10 μg (input).
Lane 2: ab215212 IP in EL4.IL-2 whole cell lysate (+)
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215212 in EL4.IL-2 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
Western blot - Anti-CD3 epsilon antibody [EPR20752] (ab215212)

All lanes : Anti-CD3 epsilon antibody [EPR20752] (ab215212) at 1/1000 dilution

Lanes 1-2 : Mouse lymph node
Lane 3 : EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-2: 3 minutes
Lane 3: 15 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] (ab215212)

Immunohistochemical analysis of Paraffin-embedded sections mouse thymus tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining on T cells in mouse thymus is observed (PMID: 18648532). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] (ab215212)

Immunohistochemical analysis of Paraffin-embedded sections mouse spleen tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining on T cells in mouse spleen is observed (PMID: 18648532). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-CD3 epsilon antibody [EPR20752] (ab215212)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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