Anti-Galanin antibody [EPR22496-254] (ab255295)
![Anti-Galanin antibody [EPR22496-254] (ab255295)](https://www.abcam.com/ps/products/255/ab255295/Images/ab255295-2-anti-galanin-antibody-epr22496-254-immunohistochemistry-pituitary-gland-human.jpg "Anti-Galanin antibody [EPR22496-254] (ab255295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22496-254] to Galanin
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Galanin antibody [EPR22496-254]
See all Galanin primary antibodies -
Description
Rabbit monoclonal [EPR22496-254] to Galanin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human pituitary gland tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22496-254 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-254] (ab255295)
Immunohistochemical analysis of paraffin-embedded human pituitary gland tissue labeling Galanin with ab255295 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255295 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-254] (ab255295)](https://www.abcam.com/ps/products/255/ab255295/Images/ab255295-1-anti-galanin-antibody-epr22496-254-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-254] (ab255295)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Galanin with ab255295 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver (PMID: 2448788) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255295 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
![Anti-Galanin antibody [EPR22496-254] (ab255295)](https://www.abcam.com/ps/products/255/ab255295/Images/ab255295-3-benefits-of-recombinant-antibodies.png)
Anti-Galanin antibody [EPR22496-254] (ab255295)
