Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22496-308] to Galanin - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Galanin antibody [EPR22496-308] - BSA and Azide free
    See all Galanin primary antibodies

  • Description

    Rabbit monoclonal [EPR22496-308] to Galanin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pituitary gland and colon tissue; mouse and rat pituitary gland tissue.

  • General notes

    Ab255700 is the carrier-free version of ab254556. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255700 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

    Immunohistochemical analysis of paraffin-embedded human pituitary gland tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254556 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on myenteric nerves of human colon (PMID: 2417157) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254556 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254556 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

    Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254556 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver (PMID: 2448788) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254556 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 2448788) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254556 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254556).

  • Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
    Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)

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