MMP12 Inhibitor Screening Assay Kit (Fluorometric) (ab139442)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
MMP12 Inhibitor Screening Assay Kit (Fluorometric)
See all MMP12 kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Abcam MMP12 Inhibitor Screening Assay Kit (Fluorometric) (ab139442) Kit is a complete assay system designed to screen MMP12 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca = (7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-ß-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.
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Notes
The kit is useful to screen inhibitors of MMP12, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 x 96 tests 96-well White Microplate 1/2 Volume 1 unit Fluorometric Assay Buffer 1 x 20ml MMP Calibration Standard 1 x 50µl MMP Fluorogenic Substrate 1 x 200µl MMP Inhibitor 1 x 50µl MMP12 Enzyme (Human, Recombinant) 1 x 14µl -
Research areas
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Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- EC 3.4.24.65
- HME
- Macrophage elastase
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Images
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Inhibition of MMP12 by NNGH (100 nM). Example of inhibitor data.