Anti-PCTP antibody [EPR15390] (ab184944)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15390] to PCTP
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-PCTP antibody [EPR15390]
See all PCTP primary antibodies -
Description
Rabbit monoclonal [EPR15390] to PCTP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and K562 cell lysates, K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15390 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PCTP antibody [EPR15390] (ab184944) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 25 kDa
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Flow cytometry analysis of paraformaldehyde-fixed K562 cells labeling PCTP with ab184944 at a 1/200 dilution (Red), with a negative control rabbit IgG (Green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.
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Western blot analysis on immunoprecipitation from Human fetal liver lysate (Lane 1), labeling PCTP using ab184944 at 1/50 dilution Lane 2 shows PBS negative control. Secondary antibody, HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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