Anti-DOK2 antibody (ab1677)
Key features and details
- Rabbit polyclonal to DOK2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DOK2 antibody
See all DOK2 primary antibodies -
Description
Rabbit polyclonal to DOK2 -
Host species
Rabbit -
Specificity
Binds to a 55-60 kDa protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
20 amino acid C-terminal peptide to p56 dok2 RasGAP-binding protein
-
Positive control
- Human U937 cells or T-cell line Jurkatt.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab1677)Ab1677 staining human breast carcinoma. Staining is localized to nucleus and cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
