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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-DOK2 (phospho Y299) antibody (ab131461)

Anti-DOK2 (phospho Y299) antibody (ab131461)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DOK2 (phospho Y299)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DOK2 (phospho Y299) antibody
    See all DOK2 primary antibodies
  • Description

    Rabbit polyclonal to DOK2 (phospho Y299)
  • Host species

    Rabbit
  • Specificity

    ab131461 detects DOK2 only when phosphorylated at Tyrosine 299.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DOK2 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • HeLa cells; K562 cell extracts; Human breast carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131461 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Other

Images

  • Western blot - Anti-DOK2 (phospho Y299) antibody (ab131461)
    Western blot - Anti-DOK2 (phospho Y299) antibody (ab131461)
    All lanes : Anti-DOK2 (phospho Y299) antibody (ab131461) at 1/500 dilution

    Lane 1 : K562 cell extracts with blocking peptide
    Lane 2 : K562 cell extracts

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (ab131461)
    Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (ab131461)

    Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling DOK2 (phospho Y299) with ab131461 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (ab131461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (ab131461)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling DOK2 (phospho Y299) with ab131461 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DOK2 (phospho Y299) antibody (ab131461)

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    Anti-DOK2 antibody (ab131488)

    Applications: ICC/IF, IHC-P, WB

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    Anti-DOK2 antibody (ab226460)

    Applications: IP, WB

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    Anti-DOK2 antibody (ab1677)

    Applications: IHC-P

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    Anti-DOK2 antibody (ab227184)

    Applications: ICC/IF, WB

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