Anti-DOK2 antibody (ab227184)
Key features and details
- Rabbit polyclonal to DOK2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOK2 antibody
See all DOK2 primary antibodies -
Description
Rabbit polyclonal to DOK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human DOK2 (internal sequence). The exact sequence is proprietary.
Database link: O60496 -
Positive control
- WB: HEK-293T, Jurkat, Raji and NCI-H929 whole cell extracts. ICC/IF: Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DOK2 antibody (ab227184)Anti-DOK2 antibody (ab227184) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Western blot - Anti-DOK2 antibody (ab227184)All lanes : Anti-DOK2 antibody (ab227184) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-DOK2 antibody (ab227184)4% paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for DOK2 (green) using ab227184 at 1/500 dilution in ICC/IF.
Nuclear Counterstain: Hoechst 33342 (blue).
