Anti-MAP4K4/NIK antibody (ab80418)
Key features and details
- Rabbit polyclonal to MAP4K4/NIK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP4K4/NIK antibody
See all MAP4K4/NIK primary antibodies -
Description
Rabbit polyclonal to MAP4K4/NIK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human MAP4K4/NIK. (AAO32626.1)
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Positive control
- Whole cell lysate from HeLa cells.
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General notes
This product was previously labelled as MAP4K4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAP4K4/NIK antibody (ab80418)All lanes : Anti-MAP4K4/NIK antibody (ab80418) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-MAP4K4/NIK antibody (ab80418)Detection of Human MAP4K4/NIK in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80418 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80418 was used. Detection was by chemiluminescence with an exposure time of 30 seconds. Lane 2 represents control IgG IP.
