Anti-PCGF5 antibody [EP19476] (ab201511)
![Anti-PCGF5 antibody [EP19476] (ab201511)](https://www.abcam.com/ps/products/201/ab201511/Images/ab201511-260696-anti-pcgf5-antibody-ep19476-western-blot.jpg "Anti-PCGF5 antibody [EP19476] (ab201511)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP19476] to PCGF5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PCGF5 antibody [EP19476]
See all PCGF5 primary antibodies -
Description
Rabbit monoclonal [EP19476] to PCGF5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse testis, ovary and thymus lysates; mES-D3, F9, C6, RAW 264.7, PC-12, NIH/3T3 and HeLa whole cell lysates; human and rat testis lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP19476 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PCGF5 antibody [EP19476] (ab201511)All lanes : Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Mouse ovary lysate
Lane 3 : Mouse thymus lysate
Lane 4 : mES-D3 (Mouse embryo stem cell line) whole cell lysate
Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PCGF5 antibody [EP19476] (ab201511)](https://www.abcam.com/ps/products/201/ab201511/Images/ab201511-260695-anti-pcgf5-antibody-ep19476-western-blot.jpg)
Western blot - Anti-PCGF5 antibody [EP19476] (ab201511)All lanes : Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : Rat testis lysate
Lane 6 : Human testis lysate
Lane 7 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 30 seconds; Lane 5-7: 3 minutes.
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![Anti-PCGF5 antibody [EP19476] (ab201511)](https://www.abcam.com/ps/products/201/ab201511/Images/ab201511-4-benefits-of-recombinant-antibodies.png)
Anti-PCGF5 antibody [EP19476] (ab201511)