C57BL/6 mouse splenocytes stained with ab237368 (right) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 μg/ml anti-CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab237368) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (1x 10⁶ in 100 μl at 1 μg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD4 PerCP conjugated antibody. Acquisition of >30,000 events were collected using a 40 mW Red laser (640 nm) and 660/20 bandpass filter. Events were gated on viable CD19 and CD3 negative lymphocytes.