Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13657] to Mad2L2/REV7
  • Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Mad2L2/REV7 antibody [EPR13657]
    See all Mad2L2/REV7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13657] to Mad2L2/REV7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, SW480, Hela, Molt-4 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: Hela cells. Flow Cyt: K562 cells IP: HeLa cell lysates

  • General notes

    This product was previously labelled as Mad2L2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13657

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Western blot - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    All lanes : Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : Molt-4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2/REV7 with ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Immunoprecipitation of HeLa cell lysate labeling Mad2L2/REV7 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

  • Flow Cytometry - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Flow Cytometry - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2/REV7 with ab180579 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/50.  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Immunohistochemical staining of paraffin-embedded human ovarian carcinoma tissue labeling Mad2L2/REV7 using ab180579 at a 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG.  Counterstained with hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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