Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD8 alpha antibody [EP1150] (ab108343)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1150] to CD8 alpha
Suitable for: IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-CD8 alpha antibody [EP1150]
See all CD8 alpha primary antibodies
Description

Rabbit monoclonal [EP1150] to CD8 alpha
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat whole cell lysate (ab7899) can be used as a positive control in WB. IHC-P: Human tonsil tissue. ICC/IF: U-937 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1150
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150] (ab108343)

ab108343 at 1/250 dilution staining CD8 alpha in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-CD8 alpha antibody [EP1150] (ab108343) This image is courtesy of an anonymous Abreview.

Flow cytometric analysis of paraformaldehyde-fixed human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab108343 at 1/40 dilution, followed by F(ab')2 Frag Goat Anti-Rabbit IgG (H+L) Alexa Fluor^® 488.

Gating Strategy: lymphocytes based on FSC vs SSC.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [EP1150] (ab108343)

Immunocytochemistry/Immunofluorescence analysis of U-937 cells labelling CD8 alpha with ab108343 at a 1/200 dilution. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were counterstained with ab195889, an Alexa Fluor^® 594-conjugated mouse anti-tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).

Secondary antibody only control: PBS instead of primary antibody.

Cytoplasmic staining seen in U-937 cells.
Anti-CD8 alpha antibody [EP1150] (ab108343)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com