Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 02, 2021

Anti-C19orf63 antibody [EPR13223] (ab181209)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13223] to C19orf63
Suitable for: Flow Cyt, WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-C19orf63 antibody [EPR13223]
See all C19orf63 primary antibodies
Description

Rabbit monoclonal [EPR13223] to C19orf63
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, A549, 293 and U87-MG cell lysates. Flow Cyt: A549 cells. ICC/IF: Ramos cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13223
Isotype

IgG
Research areas

Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)

All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EMC10 knockout HeLa cell lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Lanes 1-4: Merged signal (red and green). Green - ab181209 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab181209 Anti-C19orf63 antibody [EPR13223] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab181209 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-C19orf63 antibody [EPR13223] (ab181209)

Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling C19orf63 with ab181209 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-C19orf63 antibody [EPR13223] (ab181209)

Immunofluorescent analysis of Ramos cells (-20°C Acetone-fixed) labeling C19orf63 with ab181209 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)

All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution

Lane 1 : 293 cell lysate
Lane 2 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 27 kDa
Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)

Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution + A549 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 27 kDa
Anti-C19orf63 antibody [EPR13223] (ab181209)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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