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Anti-C19orf63 antibody [EPR13223] (ab181209)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13223] to C19orf63
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-C19orf63 antibody [EPR13223]
    See all C19orf63 primary antibodies

  • Description

    Rabbit monoclonal [EPR13223] to C19orf63

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549, 293 and U87-MG cell lysates. Flow Cyt: A549 cells. ICC/IF: Ramos cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13223

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)
    Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)
    All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : EMC10 knockout HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab181209 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab181209 Anti-C19orf63 antibody [EPR13223] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab181209 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Flow Cytometry - Anti-C19orf63 antibody [EPR13223] (ab181209)
    Flow Cytometry - Anti-C19orf63 antibody [EPR13223] (ab181209)

    Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling C19orf63 with ab181209 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-C19orf63 antibody [EPR13223] (ab181209)
    Immunocytochemistry/ Immunofluorescence - Anti-C19orf63 antibody [EPR13223] (ab181209)

    Immunofluorescent analysis of Ramos cells (-20°C Acetone-fixed) labeling C19orf63 with ab181209 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)
    Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)
    All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa

  • Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)
    Western blot - Anti-C19orf63 antibody [EPR13223] (ab181209)
    Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution + A549 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa

  • Anti-C19orf63 antibody [EPR13223] (ab181209)
    Anti-C19orf63 antibody [EPR13223] (ab181209)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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