Anti-C19orf63 antibody [EPR13223-65] (ab180148)
![Anti-C19orf63 antibody [EPR13223-65] (ab180148)](https://www.abcam.com/ps/products/180/ab180148/Images/ab180148-389448-anti-c19orf63-antibody-epr13223-65-western-blot-human-knockout-lysate.jpg "Anti-C19orf63 antibody [EPR13223-65] (ab180148)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13223-65] to C19orf63
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-C19orf63 antibody [EPR13223-65]
See all C19orf63 primary antibodies -
Description
Rabbit monoclonal [EPR13223-65] to C19orf63 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment within Human C19orf63 aa 50-150. The exact sequence is proprietary.
Database link: Q5UCC4 -
Positive control
- WB: HeLa, A549, 293 and U87-MG cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13223-65 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C19orf63 antibody [EPR13223-65] (ab180148)All lanes : Anti-C19orf63 antibody [EPR13223-65] (ab180148) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EMC10 knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaLanes 1-3: Merged signal (red and green). Green - ab180148 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.
ab180148 Anti-C19orf63 antibody [EPR13223-65] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab180148 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-C19orf63 antibody [EPR13223-65] (ab180148)](https://www.abcam.com/ps/products/180/ab180148/Images/ab180148-209941-ab180148.jpg)
Western blot - Anti-C19orf63 antibody [EPR13223-65] (ab180148)All lanes : Anti-C19orf63 antibody [EPR13223-65] (ab180148) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
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![Anti-C19orf63 antibody [EPR13223-65] (ab180148)](https://www.abcam.com/ps/products/180/ab180148/Images/ab180148-3-benefits-of-recombinant-antibodies.png)
Anti-C19orf63 antibody [EPR13223-65] (ab180148)
