Rat Uromodulin ELISA Kit (ab274405)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.9 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Uromodulin ELISA Kit
See all UMOD kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2% Inter-assay Sample n Mean SD CV% Serum 3 5.67% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.9 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 112 106% - 117% Serum 112 108% - 118% Hep Plasma 103 98% - 110% EDTA Plasma 111 105% - 117% Cit plasma 105 100% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat Uromodulin ELISA kit (ab274405) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Uromodulin protein in serum, plasmas and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Uromodulin with 6.9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Uromodulin, also known as Tamms-Horsfell protein or uromucoid, plays two distinct but important roles in biology. In the kidney, it is a key protein which forms the water-tight, yet ion-permeable, barrier that allows for proper maintaining proper serum salt balance and reabsorption. Once cleaved and released into urine, uromodulin inhibits crystal formation in the urine. Defects in this gene have been linked to kidney disease and stone formation. The immunogen used to raise the antibodies for this kit comprise the mature c-terminal portion of this protein. The N-terminal domains involved in polymerization were excluded. This protein does not share high sequence identity in mouse or human samples (87.4 and 74.5%, respectively) and, therefore, are not expected to cross-react.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat Uromodulin Capture Antibody 1 x 600µl 10X Rat Uromodulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat Uromodulin Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity. -
Tissue specificity
Synthesized by kidney. Most abundant protein in normal human urine. -
Involvement in disease
Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 1 ZP domain. -
Cellular localization
Cell membrane. Secreted. Secreted after cleavage in the urine. - Information by UniProt
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Alternative names
- ADMCKD2
- FJHN
- HNFJ
see all -
Database links
- Entrez Gene: 25128 Rat
- SwissProt: P27590 Rat
- Unigene: 31982 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat Uromodulin standard curve in 1X Cell Extraction Buffer PTR.The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Uromodulin in rat serum, plasma and urine samples.The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma - heparin 50%, plasma - citrate 100%, plasma - EDTA 100%, and urine 1:2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 3.33 ng/mL in serum, 2.22 ng/mL in plasma – heparin, 1.80 ng/mL in plasma – citrate, 2.09 ng/mL in plasma - EDTA and 1,598.60 ng/mL in urine.
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Sandwich ELISA - Rat Uromodulin ELISA Kit (ab274405)To learn more about the advantages of recombinant antibodies see here.
