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Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MSK1T581-A5] to MSK1 (phospho T581)
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MSK1 (phospho T581) antibody [MSK1T581-A5]
    See all MSK1 primary antibodies

  • Description

    Rabbit monoclonal [MSK1T581-A5] to MSK1 (phospho T581)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MSK1 (phospho T581). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O75582

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T (treated with UV) cell extract; Flow cyt: U937 cells (treated with CalA); K562 and C6 cells (both treated with pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)

    Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or pervanadate-treated and stained using 0.1µg/mL isotype control (orange) or pervanadate-treated and stained using 0.1µg/mL ab278550 (green) or pervanadate and blocked with phospho peptide (gold) or pervanadate and blocked with non-phospho peptide (purple).

  • Western blot - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Western blot - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Western blot analysis of 293T cell extract untreated or treated with staurosporine using 0.1 µg/mL ab278550.
  • Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with CalA (green) using ab278550 at 1.0 µg/mL.
  • Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) ab278550 at 0.1µg/mL. .
  • Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Flow Cytometry - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)

    Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or treated with imatinib (red) or with pervanadate (green) using ab278550 at 0.1 µg/mL. 

  • Western blot - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Western blot - Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    All lanes : Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550) at 0.1 µg/ml

    Lane 1 : Human epithelial cell line from embryonic kidney transformed with large T antigen (293T cells) cell extract (treated with UV)
    Lane 2 : Human epithelial cell line from embryonic kidney transformed with large T antigen (293T cells) cell extract (untreated)

    Predicted band size: 90 kDa

  • Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)
    Anti-MSK1 (phospho T581) antibody [MSK1T581-A5] (ab278550)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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