Anti-SGK1 antibody (ab59337)
Key features and details
- Rabbit polyclonal to SGK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SGK1 antibody
See all SGK1 primary antibodies -
Description
Rabbit polyclonal to SGK1 -
Host species
Rabbit -
Specificity
Detects endogenous levels of total SGK protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized non-phosphopeptide derived from human SGK1 around the phosphorylation site of serine 78 (P-P-SP-P-S).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SGK1 antibody (ab59337)All lanes : Anti-SGK1 antibody (ab59337) at 1/500 dilution
Lane 1 : 293 cells treated
with heat shock
Lane 2 : 293 cells treated
with heat shock with immunizing non-phosphopeptide
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-SGK1 antibody (ab59337)ICC/IF image of ab59337 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59337, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody (ab59337)ab59337, at a 1/50-1/100 dilution, staining SGK1 in Human brain sections. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody (ab59337)ab59337, at a 1/50-1/100 dilution, staining SGK1 in Human brain sections, in the presence of the immunizing peptide.
