Anti-SGK1 (phospho S422) antibody (ab55281)
Key features and details
- Rabbit polyclonal to SGK1 (phospho S422)
- Suitable for: WB, IHC-P, ELISA, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SGK1 (phospho S422) antibody
See all SGK1 primary antibodies -
Description
Rabbit polyclonal to SGK1 (phospho S422) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human SGK1 (phospho S422).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SGK1 (phospho S422) antibody (ab55281)All lanes : Anti-SGK1 (phospho S422) antibody (ab55281) at 1/500 dilution
Lane 1 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes) plus immunizing phosphopeptide
Lane 2 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes)
Developed using the ECL technique.
Predicted band size: 49 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 (phospho S422) antibody (ab55281)Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using ab55281 at a 1/50 diluton.
Left: No phosphopeptide.
Right: Sample treated with immunizing phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-SGK1 (phospho S422) antibody (ab55281)ICC/IF image of ab55281 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% PFA for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55281, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
