Human EIF2AK2 (PKR) knockout A549 cell lysate (ab256901)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262037 - Human EIF2AK2 knockout A549 cell lysate 1 x 100µg ab255554 - Human wild-type A549 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human EIF2AK2 knockout A549 cell lysate (ab256901)
Lane 1:Wild-type A549 cell lysate (20 ug)
Lane 2:EIF2AK2 knockout A549 cell lysate (20 ug)
Lane 3:K-562 cell lysate (20 ug)ab184257 was shown to specifically react with PKR in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267000 (knockout cell lysate ab256901) was used. Wild-type and PKR knockout samples were subjected to SDS-PAGE. ab184257 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human EIF2AK2 knockout A549 cell lysate (ab256901)Lane 1:Wild-type A549 cell lysate (20 ug)
Lane 2:EIF2AK2 knockout A549 cell lysate (20 ug)
Lane 3:K-562 cell lysate (20 ug)ab32052 was shown to specifically react with PKR in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267000 (knockout cell lysate ab256901) was used. Wild-type and PKR knockout samples were subjected to SDS-PAGE. ab32052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human EIF2AK2 knockout A549 cell lysate (ab256901)Allele-1: 10 bp deletion in exon5
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Sanger Sequencing - Human EIF2AK2 knockout A549 cell lysate (ab256901)Allele-2: 1 bp deletion in exon5
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Sanger Sequencing - Human EIF2AK2 knockout A549 cell lysate (ab256901)Allele-3: 1 bp insertion in exon5
