Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

1 ml 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [80] to pan Keratin
Suitable for: Flow Cyt, WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-pan Keratin antibody [80]
See all pan Keratin primary antibodies
Description

Mouse monoclonal [80] to pan Keratin
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Callus cytokeratins isolated from fresh human skin tissue
Positive control
- Human callus or keratinocytes

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.3
Preservative: 0.07% Sodium azide
Constituent: 1% Fetal calf serum
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

80
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)

ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068) This image is courtesy of an anonymous Abreview

Ab8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor^® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.
See Abreview
Western blot - Anti-pan Keratin antibody [80] (ab8068)

Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 30 seconds
Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)

Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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