Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free
    See all Tyrosine Hydroxylase primary antibodies

  • Description

    Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Mouse
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human adrenal gland lysate. SH-SY5Y whole cell lysate. Mouse and rat brain lysate. PC-12 whole cell lysate. IHC-P: Human and mouse cerebral cortex tissue, Rat and mouse brain tissue. ICC: Neuro-2a and mouse midbrain neuron cells. Flow Cyt: SH-SY5Y cells.

  • General notes

    Ab219729 is the carrier-free version of ab75875. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab219729 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    All lanes : Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) at 1/1000 dilution (Purified)

    Lane 1 : Human adrenal gland lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using ab75875, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)

    This IHC data was generated using the same anti-Tyrosine Hydroxylase antibody clone, EP1533Y, in a different buffer formulation (cat# ab75875).

    Immunohistochemical analysis of rat brain tissue section labeling Tyrosine Hydroxylase with ab75875 at 1/1000 dilution. The tissue was fixed with Formaldehyde. Antigen retrieval was heat mediated using citric acid. A biotin conjugated goat anti-rabbit polyclonal IgG at 1/250 was used as the secondary antibody.

  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)

    This data was developed using ab75875, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse midbrain neuron cells labelling Tyrosine Hydroxylase with ab75875 at 1/10 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary midbrain neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    This data was developed using ab75875, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/50 dilution (2.18 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Flow Cytometry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    This data was developed using ab75875, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    This data was developed using ab75875, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with purified ab75875 at 1/1500 dilution (0.07 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729) This image is courtesy of an Abreview submitted by Carl Hobbs.

    Immunohistochemical analysis of mouse brain tissue section labeling Tyrosine Hydroxylase with ab75875 at 1/800 dilution. The tissue was fixed with Formaldehyde. Antigen retrieval was heat mediated using citric acid. A biotin conjugated goat anti-rabbit polyclonal IgG at 1/250 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75875).

  • Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
    Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)

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