Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab117112)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody - Neuronal Marker
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Rat
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Immunogen
Synthetic peptide corresponding to Human Tyrosine Hydroxylase aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139516)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P RatAll applications MouseDogHumanChimpanzeeMacaque monkeyGorillaChinese hamsterOrangutanApplication Abreviews Notes IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab117112)IHC image of Tyrosine 3-monooxygenase staining in sagittal 6 week rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117112, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References (3)
ab117112 has been referenced in 3 publications.
- Du T et al. Injection of a-syn-98 Aggregates Into the Brain Triggers a-Synuclein Pathology and an Inflammatory Response. Front Mol Neurosci 12:189 (2019). PubMed: 31447645
- Chen WJ et al. The Impact of Renal Denervation on the Progression of Heart Failure in a Canine Model Induced by Right Ventricular Rapid Pacing. Front Physiol 10:1625 (2019). PubMed: 32082182
- Yun JW et al. Behavior, PET and histology in novel regimen of MPTP marmoset model of Parkinson's disease for long-term stem cell therapy. Tissue Eng Regen Med 13:100-109 (2016). PubMed: 30603390
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab117112)IHC image of Tyrosine 3-monooxygenase staining in sagittal 6 week rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117112, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
