Anti-RNA polymerase II (phospho S5) antibody (ab240740)
Key features and details
- Rabbit polyclonal to RNA polymerase II (phospho S5)
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RNA polymerase II (phospho S5) antibody
See all RNA polymerase II primary antibodies -
Description
Rabbit polyclonal to RNA polymerase II (phospho S5) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Human RNA polymerase II (phospho S5). Phosphorylated synthetic peptide, which represents a portion of human RNA Polymerase II around serine 5 of the C-terminal repeat YSPTSPS using the numbering given in entry NP_000928.1 (GeneID 5430).
Database link: P24928 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human Ovarian carcinoma and mouse renal carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to RNA polymerase II (phospho S5) immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNA polymerase II (phospho S5) antibody (ab240740)All lanes : Anti-RNA polymerase II (phospho S5) antibody (ab240740) at 0.02 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. Treated with phosphatase (+).
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. Mock treated (-).
Lysates/proteins at 50 µg per lane.
Predicted band size: 217 kDaTo examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used (lower panel).
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II (phospho S5) antibody (ab240740)Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for RNA polymerase II (phospho S5) using ab240740 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-RNA polymerase II (phospho S5) antibody (ab240740)RNA polymerase II (phospho S5) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab240740 at 1 μg per reaction. Western blot was performed on the immunoprecipitate using ab240740 at 0.02 μg/mL.
Lane 1: ab240740 IP in HeLa whole cell lysate, with phosphatase treatment (+).
Lane 2: ab240740 IP in HeLa whole cell lysate, without phosphatase treatment (-).
Lane 3: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II (phospho S5) antibody (ab240740)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for RNA polymerase II (phospho S5) using ab240740 at 1/200 dilution in immunohistochemical analysis. DAB staining.
