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HRP Anti-beta Actin antibody [AC-15] (ab49900)

Key features and details

  • HRP Mouse monoclonal [AC-15] to beta Actin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-beta Actin antibody [AC-15]
    See all beta Actin primary antibodies

  • Description

    HRP Mouse monoclonal [AC-15] to beta Actin

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey
    Predicted to work with: a wide range of other speciesDoes not react with: Drosophila melanogaster, Dictyostelium discoideum

  • Immunogen

    Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    DDDIAALVIDNGSGK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • NIH 3T3 whole cell lysate (ab7179) can be used as a positive control in WB. Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates

Properties

Images

  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    All lanes : HRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/50000 dilution

    Lane 1 : A549 Lysate
    Lane 2 : HeLa Lysate
    Lane 3 : 3T3 Lysate
    Lane 4 : NRK Lysate
    Lane 5 : MDCK Lysate
    Lane 6 : MDBK Lysate
    Lane 7 : BHK Lysate
    Lane 8 : COS7 Lysate
    Lane 9 : CFB Lysate

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900) Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

    Normalization of results was ensured by running parallel western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p

  • Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
    Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900) This image is courtesy of an anonymous Abreview
    HRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa



    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

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