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Signal Transduction Antibodies beta actin

Anti-beta Actin antibody [8F10-G10] (ab170325)

Price and availability

268 032 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-beta Actin antibody [8F10-G10] (ab170325)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8F10-G10] to beta Actin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-beta Actin antibody [8F10-G10]
    See all beta Actin primary antibodies
  • Description

    Mouse monoclonal [8F10-G10] to beta Actin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Rabbit
    Goat
    Human
    Saccharomyces cerevisiae
    Drosophila melanogaster
    Zebrafish
    African green monkey
    Chinese hamster
    See all applications and species data
  • Immunogen

    Full length protein corresponding to Human beta Actin. Expressed in E.coli.
    Database link: NM_001101

  • Positive control

    • WB: COS7, K562, HeLa, 3T3 cell lysate.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Concentrated Culture Supernatant
  • Clonality

    Monoclonal
  • Clone number

    8F10-G10
  • Isotype

    IgG2b
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Images

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 3T3 cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution

    Lane 1 : Goat muscle cell lysate
    Lane 2 : CHO-K1 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : 3T3 cell lysate
    Lane 6 : K562 cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes :

    Lane 1 : Rabbit spleen cell lysate
    Lane 2 : Rabbit colon cell lysate
    Lane 3 : Rabbit skeletal muscle cell lysate
    Lane 4 : Rabbit liver cell lysate
    Lane 5 : Rabbit heart cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
    Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)

    All lanes : Zebrafish row cell lysate

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution

    Lane 1 : Arabidopsis cell lysate at 30 µg
    Lane 2 : Arabidopsis cell lysate at 15 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution

    Lane 1 : S. cerevisiae cell lysate at 10 µg
    Lane 2 : P. pastoris cell lysate at 10 µg
    Lane 3 : S. cerevisiae cell lysate at 5 µg
    Lane 4 : P. pastoris cell lysate at 5 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes :

    Lane 1 : COS7 cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : k562 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
    All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution

    Lane 1 : Drosophila melanogaster cell lysate at 40 µg
    Lane 2 : Drosophila melanogaster cell lysate at 20 µg

    Predicted band size: 42 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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