Anti-beta Actin antibody [8F10-G10] (ab170325)
Key features and details
- Mouse monoclonal [8F10-G10] to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
- Isotype: IgG2b
Overview
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Product name
Anti-beta Actin antibody [8F10-G10]
See all beta Actin primary antibodies -
Description
Mouse monoclonal [8F10-G10] to beta Actin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB RabbitGoatHumanSaccharomyces cerevisiaeDrosophila melanogasterZebrafishAfrican green monkeyChinese hamster -
Immunogen
Full length protein corresponding to Human beta Actin. Expressed in E.coli.
Database link: NM_001101 -
Positive control
- WB: COS7, K562, HeLa, 3T3 cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
8F10-G10 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : COS7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 3T3 cell lysate
Predicted band size: 42 kDa
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution
Lane 1 : Goat muscle cell lysate
Lane 2 : CHO-K1 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : 3T3 cell lysate
Lane 6 : K562 cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes :
Lane 1 : Rabbit spleen cell lysate
Lane 2 : Rabbit colon cell lysate
Lane 3 : Rabbit skeletal muscle cell lysate
Lane 4 : Rabbit liver cell lysate
Lane 5 : Rabbit heart cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes : Zebrafish row cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
Lane 1 : Arabidopsis cell lysate at 30 µg
Lane 2 : Arabidopsis cell lysate at 15 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : S. cerevisiae cell lysate at 10 µg
Lane 2 : P. pastoris cell lysate at 10 µg
Lane 3 : S. cerevisiae cell lysate at 5 µg
Lane 4 : P. pastoris cell lysate at 5 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
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All lanes :
Lane 1 : COS7 cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : k562 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 42 kDa
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 1 : Drosophila melanogaster cell lysate at 40 µg
Lane 2 : Drosophila melanogaster cell lysate at 20 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?