HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
![HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29577-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg "HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Key features and details
- HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control
See all beta Actin primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)).
(Peptide available asab13772) -
Positive control
- HeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 8226 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : human testis lysate
Lane 2 : human brain lysate
Lane 3 : human tonsil lysate
Lane 4 : mouse brain lysate
Lane 5 : mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Exposure time: 2 minutes
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![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29576-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 3T3 whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds
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![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.com/ps/products/20/ab20272/Images/ab20272-29575-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)Lanes 1-4 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
Lanes 5-8 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lanes 3 & 7 :NIH/3T3 whole cell lysate (ab7179)
Lanes 4 & 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
