Anti-beta III Tubulin antibody - C-terminal (ab230847)
Key features and details
- Rabbit polyclonal to beta III Tubulin - C-terminal
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-beta III Tubulin antibody - C-terminal
See all beta III Tubulin primary antibodies -
Description
Rabbit polyclonal to beta III Tubulin - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee
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Immunogen
Synthetic peptide within Human beta III Tubulin (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q13509 -
Positive control
- WB: Mouse brain tissue extract; rat brain tissue extract; HeLa and SK-N-SH whole cell extracts. IHC-Fr: E13.5 rat brain tissue. ICC/IF: SK-N-SH cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal (ab230847)
Frozen sectioned E13.5 rat brain tissue stained for beta III Tubulin (green) with ab230847 at 1/250 dilution in immunohistochemical analysis.
Blue: Fluoroshield with DAPI
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal (ab230847)SK-N-SH (human neuroblastoma cell line) cells stained for beta III Tubulin (green) using ab230847 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Western blot - Anti-beta III Tubulin antibody - C-terminal (ab230847)All lanes : Anti-beta III Tubulin antibody - C-terminal (ab230847) at 1/10000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
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Western blot - Anti-beta III Tubulin antibody - C-terminal (ab230847)All lanes : Anti-beta III Tubulin antibody - C-terminal (ab230847) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa10% SDS-PAGE
